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pExodus CMV.Artemis
(Plasmid #40211)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40211 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    In-house
  • Backbone size w/o insert (bp) 4442
  • Modifications to backbone
    Multiple cloning site, CMV and T7 promoters
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Artemis
  • Alt name
    SNM1c
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2085
  • Entrez Gene
    DCLRE1C (a.k.a. A-SCID, DCLREC1C, RS-SCID, SCIDA, SNM1C)
  • Promoter CMV and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site EcoR1 (destroyed during cloning)
  • 5′ sequencing primer T7 FP
  • 3′ sequencing primer BGH RP
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received the Trex2 ORF-containing construct from the laboratory of Daniel Stetson, University of Washington
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExodus CMV.Artemis was a gift from Andrew Scharenberg (Addgene plasmid # 40211 ; http://n2t.net/addgene:40211 ; RRID:Addgene_40211)
  • For your References section:

    Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, Paques F, Rawlings DJ, Scharenberg AM. Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 2012 Sep 2. 10.1038/nmeth.2177 PubMed 22941364