|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40222||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6949
- Total vector size (bp) 9200
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2175
Mutationamino acids 459-460 replaced with asparagine
Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti4/V5-DEST-P85(KS459del) was a gift from Lynda Chin (Addgene plasmid # 40222 ; http://n2t.net/addgene:40222 ; RRID:Addgene_40222)
Map uploaded by the depositor.