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pcDNA3-mRuby2
(Plasmid #40260)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40260 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 40260-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5478
  • Total vector size (bp) 6192
  • Modifications to backbone
    His6 and Xpress tags at N-terminus of insert.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRuby2
  • Species
    Synthetic; Entacmaea quadricolor
  • Insert Size (bp)
    714
  • Promoter CMV IE
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

mRuby2 is the brightest red fluorescent protein (RFP) characterized to date, with robust performance in protein fusions. Also, it is useful as FRET acceptor for Clover GFP in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap.

Information for Cloning Grade DNA (Catalog # 40260-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mRuby2 was a gift from Michael Lin (Addgene plasmid # 40260 ; http://n2t.net/addgene:40260 ; RRID:Addgene_40260)
  • For your References section:

    Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245