PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40260||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5478
- Total vector size (bp) 6192
Modifications to backboneHis6 and Xpress tags at N-terminus of insert.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesSynthetic; Entacmaea quadricolor
Insert Size (bp)714
- Promoter CMV IE
/ Fusion Proteins
- His6 (N terminal on backbone)
- Xpress (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Rev (Common Sequencing Primers)
mRuby2 is the brightest red fluorescent protein (RFP) characterized to date, with robust performance in protein fusions. Also, it is useful as FRET acceptor for Clover GFP in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-mRuby2 was a gift from Michael Lin (Addgene plasmid # 40260 ; http://n2t.net/addgene:40260 ; RRID:Addgene_40260)
For your References section:Improving FRET dynamic range with bright green and red fluorescent proteins. Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. 10.1038/nmeth.2171 PubMed 22961245