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Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)
(Plasmid #40340)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40340 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Blimp1 promoter
  • Alt name
    PRDM1 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • GenBank ID
    NG_029115.1
  • Entrez Gene
    PRDM1 (a.k.a. BLIMP1, PRDI-BF1)
  • Promoter Blimp1
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A 2000-bp region of the human Blimp-1 promoter was isolated from human (Raji cell) genomic DNA by PCR using 5-ATTTTAGGGTTGCCTGGCTAAGCC-3 as the forward and 5-GTCACGGCAGCACTTTGTCTGTGT-3 as the reverse primers. The PCR product obtained was ligated into the pcR2.1 TOPO cloning vector (Invitrogen, Carlsbad, CA) and then subcloned into pGL3-basic (Promega, Madison, WI) using SacI and XhoI sites. The promoter construct was sequenced to verify that the proper sequence was amplified.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40340 ; http://n2t.net/addgene:40340 ; RRID:Addgene_40340)
  • For your References section:

    Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. Vasanwala FH, Kusam S, Toney LM, Dent AL. J Immunol. 2002 Aug 15;169(4):1922-9. PubMed 12165517