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Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)
(Plasmid #40341)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40341 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Blimp1 promoter (mutated)
  • Alt name
    PRDM1 promoter (mutated)
  • Species
    H. sapiens (human)
  • Mutation
    Two AP-1 sites at -1813 and -1647 replaced with BamHI and EcoRI restriction enzyme sites, respectively
  • GenBank ID
  • Promoter Blimp1
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This mutant AP-1 site promoter, Mut.Blimp-1pGL3, was constructed by replacing the two AP-1 sites at -1813 and -1647 with BamHI and EcoRI restriction enzyme sites, respectively.

Addgene sequencing results have determined that this is a truncated promoter of ~400bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40341 ; http://n2t.net/addgene:40341 ; RRID:Addgene_40341)
  • For your References section:

    Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. Vasanwala FH, Kusam S, Toney LM, Dent AL. J Immunol. 2002 Aug 15;169(4):1922-9. PubMed 12165517