Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)
(Plasmid #40341)

• Depositing Lab: Alexander Dent
• Publication: Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 6818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Blimp1 promoter (mutated)
• Alt name: PRDM1 promoter (mutated)
• Species: H. sapiens (human)
• Mutation: Two AP-1 sites at -1813 and -1647 replaced with BamHI and EcoRI restriction enzyme sites, respectively
• GenBank ID:
• Entrez Gene: PRDM1 (a.k.a. BLIMP1, PRDI-BF1)
• Promoter: Blimp1
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This mutant AP-1 site promoter, Mut.Blimp-1pGL3, was constructed by replacing the two AP-1 sites at -1813 and -1647 with BamHI and EcoRI restriction enzyme sites, respectively.

Addgene sequencing results have determined that this is a truncated promoter of ~400bp