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pET11a-Z-NspGFP
(Plasmid #40729)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40729 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 5641
  • Total vector size (bp) 6256
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N super positive GFP - Leucine Zipper
  • Alt name
    NspGFP-Z
  • Species
    Synthetic
  • Insert Size (bp)
    615
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11a-Z-NspGFP was a gift from Brian McNaughton (Addgene plasmid # 40729 ; http://n2t.net/addgene:40729 ; RRID:Addgene_40729)
  • For your References section:

    Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo. Blakeley BD, Chapman AM, McNaughton BR. Mol Biosyst. 2012 Aug;8(8):2036-40. Epub 2012 Jun 12. 10.1039/c2mb25130b PubMed 22692102