PurposeRenilla luciferase reporter containing siRNA target sites
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40762||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3 copies of the CXCR4 small RNA target site
- Promoter TK/SV40
/ Fusion Protein
- luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CCAATGCTATTGTTGAAGGTGCCAAG
- 3′ sequencing primer TCAGACAAACCCTAACCACCGC (Common Sequencing Primers)
This plasmid also contains a firefly reporter cassette that has been specifically designed to be an intraplasmid transfection normalization reporter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCheck2 3xUTR was a gift from Phillip Zamore (Addgene plasmid # 40762 ; http://n2t.net/addgene:40762 ; RRID:Addgene_40762)
For your References section:Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing. Broderick JA, Salomon WE, Ryder SP, Aronin N, Zamore PD. RNA. 2011 Oct;17(10):1858-69. Epub 2011 Aug 30. 10.1261/rna.2778911 PubMed 21878547