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pSPORT-RREB1
(Plasmid #41145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41145 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 41145-D50 50 μg of DNA in Tris buffer $495
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DNA 41145-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSPORT6.1
  • Backbone size w/o insert (bp) 4149
  • Total vector size (bp) 9404
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RREB-1
  • Alt name
    RREB1
  • Alt name
    hZIP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5255
  • GenBank ID
    NP_001003698.1
  • Entrez Gene
    RREB1 (a.k.a. FINB, HNT, LZ321, RREB-1, Zep-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains the G195R natural variant in RREB1

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPORT-RREB1 was a gift from Joshua Mendell (Addgene plasmid # 41145 ; http://n2t.net/addgene:41145 ; RRID:Addgene_41145)

  • For your References section:

    RREB1 repressed miR-143/145 modulates KRAS signaling through downregulation of multiple targets. Kent OA, Fox-Talbot K, Halushka MK. Oncogene. 2012 Jul 2. doi: 10.1038/onc.2012.266. 10.1038/onc.2012.266 PubMed 22751122
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