pACYC-T7
(Plasmid #41187)

• Depositing Lab: Dan Bolon
• Publication: Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-95. Epub 2007 Oct 1.

Backbone

• Vector backbone: pACYC-Duet1 and pET21
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 6500
• Modifications to backbone: Multiple cloning site was altered to remove restriction sites. Some un-necessary parts of the vector were removed to make a compact plasmid.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S. cerevisiae Hsp82
• Alt name: Hsp90
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2130
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry