pETcon(I-LtrI)
(Plasmid #41523)

• Depositing Lab: Andrew Scharenberg
• Publication: pETCON Yeast Surface Display Plasmids (unpublished)

Backbone

• Vector backbone: pETcon
• Backbone size w/o insert (bp): 6145
• Total vector size (bp): 7069
• Modifications to backbone: Inserted yeast codon-optimized I-LtrI homing endonuclease using NdeI/XhoI cloning sites.
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: I-LtrI
• Alt name: I-LtrI homing endonuclease
• Species: Leptographium truncatum
• Insert Size (bp): 924
• GenBank ID: FJ717852.1
• Promoter: GAL1
• Tags / Fusion Proteins:
  • HA Tag (N terminal on backbone)
  • 3x(G4S) Linker (N terminal on backbone)
  • G3S Linker (C terminal on backbone)
  • Myc Tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAL1 Promoter
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry