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pCAGGS-Flag-hsDicer (Y971A/Y972A)
(Plasmid #41590)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4790
  • Vector type
    Mammalian Expression
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    hsDicer
  • Alt name
    Dicer1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5796
  • Mutation
    Y971A/Y972A
  • GenBank ID
    NM_177438 NM_030621
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, HERNA, MNG1, RMSE2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-Flag-hsDicer (Y971A/Y972A) was a gift from Phil Sharp (Addgene plasmid # 41590)
  • For your References section:

    In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. Gurtan AM, Lu V, Bhutkar A, Sharp PA. RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30. 10.1261/rna.032680.112 PubMed 22546613