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pSKDuet16
(Plasmid #41684)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41684 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    novagen
  • Total vector size (bp) 4392
  • Modifications to backbone
    removed the second MCS of pRSFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE intein
  • Alt name
    cis-splicing DnaE intein with GB1 as exteins
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    825
  • Promoter T7
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKDuet16 was a gift from Hideo Iwai (Addgene plasmid # 41684 ; http://n2t.net/addgene:41684 ; RRID:Addgene_41684)
  • For your References section:

    Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. Ellila S, Jurvansuu JM, Iwai H. FEBS Lett. 2011 Oct 10. 10.1016/j.febslet.2011.10.005 PubMed 22001202