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tol2-4xnr UAS-PA Rac1-mCherry
(Plasmid #41878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41878 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    photoactivatable Rac1
  • Alt name
    PA-Rac1
  • Species
    H. sapiens (human)
  • Mutation
    Rac1 starts at I4 and contains mutations Q61L, E91H and N92H (Rac1 insert derived from Addgene plasmid# 22027)
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Tags / Fusion Proteins
    • 6x His (N terminal on insert)
    • mCherry (N terminal on insert)

Resource Information

Depositor Comments

Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory at bp# 895, 920 and 1702. According to the depositing lab, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tol2-4xnr UAS-PA Rac1-mCherry was a gift from Anna Huttenlocher (Addgene plasmid # 41878 ; http://n2t.net/addgene:41878 ; RRID:Addgene_41878)