tol2-4xnr UAS-PA Rac1-mCherry
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41878||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5473
Vector typezebrafish expression
Growth in Bacteria
Gene/Insert namephotoactivatable Rac1
SpeciesH. sapiens (human)
MutationRac1 starts at I4 and contains mutations Q61L, E91H and N92H (Rac1 insert derived from Addgene plasmid# 22027)
Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
/ Fusion Proteins
- 6x His (N terminal on insert)
- mCherry (N terminal on insert)
Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory at bp# 895, 920 and 1702. According to the depositing lab, these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:tol2-4xnr UAS-PA Rac1-mCherry was a gift from Anna Huttenlocher (Addgene plasmid # 41878 ; http://n2t.net/addgene:41878 ; RRID:Addgene_41878)