pCGMbX
(Plasmid #42300)

• Purpose: (Empty Backbone)
• Depositing Lab: Steven Almo
• Publication: inducible Drosophila S2 vector (unpublished)

Backbone

• Vector backbone: pMT/BiP/V5-His A
• Backbone manufacturer: Invitrogen (Life Technologies)
• Backbone size (bp): 3600
• Modifications to backbone: Expression cassette inserted upstream of the metallothionein promoter, enabling constitutive expression of eGFP.
• Vector type: Insect Expression
• Promoter: metallothonein
• Tags / Fusion Proteins:
  • BiP signal sequence (N terminal on insert)
  • V5-epitope (C terminal on backbone)
  • His6 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: catctcagtgcaactaaa
• 3′ sequencing primer: TGTCTGGATCCTCGATCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid was generated from a combination of pCo-Blast (Invitrogen), pMT/BiP/V5-His A (Invitrogen), and pMT-eGFP by Vladimir Vigdorovich.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry