|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42581||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6119
Vector typeMammalian Expression, AAV ; Adeno-associated Virus ()
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsBackbone contains ccdb gene, serve as a negative selection cassette. Please use ccdb resistant strain for maintenance of the plasmid.
Copy numberHigh Copy
Alt nameTALE Backbone
Alt nameTALE VP64
Insert Size (bp)2015
- Promoter CAG hybrid
/ Fusion Protein
- 3XFlag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI/BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CAG-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece. Please refer to our website at http://taleffector.genome-engineering.org/ for more information on these new backbone vectors in the 'Protocols - Reagents' section under 'Special Note for New Adeno-associated Virus (AAV) TALE-TF and TALEN Backbones'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV_TALE-TF(VP64)-BB_V3 was a gift from Feng Zhang (Addgene plasmid # 42581 ; http://n2t.net/addgene:42581 ; RRID:Addgene_42581)
For your References section:Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628