|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42593||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerStanley Lemon (PubMed ID 10611164)
- Total vector size (bp) 8695
Vector typeMammalian Expression, Luciferase
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehairpin - BCL2 5'UTR - firefly luciferase
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)3000
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
phpBCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details.
This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits the ability of the BCL2 5'UTR to mediate translation of firefly luciferase.
Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:phpBCL2-FL(pA) was a gift from Richard Lloyd (Addgene plasmid # 42593 ; http://n2t.net/addgene:42593 ; RRID:Addgene_42593)
For your References section:BCL-2 translation is mediated via internal ribosome entry during cell stress. Sherrill KW, Byrd MP, Van Eden ME, Lloyd RE. J Biol Chem. 2004 Jul 9;279(28):29066-74. Epub 2004 May 3. 10.1074/jbc.M402727200 PubMed 15123638