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CLPIT Cry2PHR-mCh-Rac1
(Plasmid #42955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42955 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CLPIT
  • Backbone size w/o insert (bp) 7792
  • Total vector size (bp) 10623
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry2PHR-mCherry-Rac1
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2832
  • Entrez Gene
    RAC1 (a.k.a. MIG5, Rac-1, TC-25, p21-Rac1)
  • Promoter Tet-Off promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer AGACGCCATCCAACGCTGTTT
  • 3′ sequencing primer AGAGCCTGGACCACTGATATCCTGTCT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CLPIT Cry2PHR-mCh-Rac1 was a gift from David Schaffer (Addgene plasmid # 42955)
  • For your References section:

    Optogenetic protein clustering and signaling activation in mammalian cells. Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV. Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360. 10.1038/nmeth.2360 PubMed 23377377