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pCFJ1200
(Plasmid #44485)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44485 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2305
  • Vector type
    Multiple cloning site vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Minimal Mos1, PuroR, peel-1, MCS
  • Species
    D. melanogaster (fly), C. elegans (nematode); Bacterial
  • Insert Size (bp)
    3966

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer M13rev
  • 3′ sequencing primer M13-fwd20
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ1200 was a gift from Erik Jorgensen (Addgene plasmid # 44485 ; http://n2t.net/addgene:44485 ; RRID:Addgene_44485)
  • For your References section:

    Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376