|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44650||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Gene/Insert nameTurbo Sapphire
Terms and Licenses
- Not Available to Industry
This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
yETSapphire was generated by mutagenizing yEGFP to the sequence published in "Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP." Zapata-Hommer O, Griesbeck O. BMC Biotechnol. 2003 May 22;3:5. Epub 2003 May 22.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-link-yETSapphire-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44650 ; http://n2t.net/addgene:44650 ; RRID:Addgene_44650)