pCMV-BD-cop1at(335-675)
(Plasmid #44973)

• Depositing Lab: Thanos Halazonetis
• Publication: Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.

Backbone

• Vector backbone: pCMV-BD
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4251
• Total vector size (bp): 5730
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BD-COP1
• Alt name: CONSTITUTIVE PHOTOMORPHOGENIC 1
• Alt name: E3 ubiquitin-protein ligase COP1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1479
• Mutation: deleted amino acids 1-334
• GenBank ID: NM_128855.3
• Entrez Gene: COP1 (a.k.a. AT2G32950, ARABIDOPSIS THALIANA CONSTITUTIVE PHOTOMORPHOGENIC 1, ATCOP1, CONSTITUTIVE PHOTOMORPHOGENIC 1, DEETIOLATED MUTANT 340, DET340, EMB168, EMBRYO DEFECTIVE 168, FUS1, FUSCA 1, T21L14.11, T21L14_11)
• Promoter: pCMV
• Tag / Fusion Protein:
  • GAL4 DNA-binding domain (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry