pCMV-AD-uvr8at
(Plasmid
#44971)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-AD
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3555
- Total vector size (bp) 5484
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAD-UVR8
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Alt nameUV-B resistance 8
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1929
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Mutationdeleted Methionine 1
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GenBank IDNM_125781.3
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Entrez GeneUVR8 (a.k.a. AT5G63860, MGI19.7, MGI19_7, UVB-RESISTANCE 8)
- Promoter pCMV
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Tag
/ Fusion Protein
- NF-κB activation domain (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-AD-uvr8at was a gift from Thanos Halazonetis (Addgene plasmid # 44971 ; http://n2t.net/addgene:44971 ; RRID:Addgene_44971) -
For your References section:
Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. Crefcoeur RP, Yin R, Ulm R, Halazonetis TD. Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800. 10.1038/ncomms2800 PubMed 23653191
