|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45050||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
- Promoter TPI
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
additional information can be found at:
The depositor mtGFP insert seq shows 5' HindIII and a 3' EcoRI, they are actually reversed in this construct, the cloning information displayed is correct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYX142-mtGFP was a gift from Benedikt Westermann (Addgene plasmid # 45050)
For your References section:Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Westermann B, Neupert W. Yeast. 2000 Nov;16(15):1421-7. 10.1002/1097-0061(200011)16:15<1421::AID-YEA624>3.0.CO;2-U PubMed 11054823
Map uploaded by the depositor.