Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45174||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5739
Modifications to backboneThe original pBR322 Origin of Replication from pET-28a(+) was replaced with the p15A Origin of Replication.
Vector typeBacterial Expression
- Promoter T7
/ Fusion Proteins
- 6xHis/Thrombin/T7 (N terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
Copy numberLow Copy
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer 5' - TAA TAC GAC TCA CTA TAG GG - 3'
- 3′ sequencing primer 5' - GCT AGT TAT TGC TCA GCG G - 3' (Common Sequencing Primers)
In Sathiamoorthy et al, there is an error in the title of one of the supplementary figures. Figure S3 should read "Construction of pSAMRNAI" and in the figure the final constructed vector is "pSAMRNAI".
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSAM was a gift from Jumi Shin (Addgene plasmid # 45174 ; http://n2t.net/addgene:45174 ; RRID:Addgene_45174)
For your References section:Boundaries of the origin of replication: creation of a pET-28a-derived vector with p15A copy control allowing compatible coexistence with pET vectors. Sathiamoorthy S, Shin JA. PLoS One. 2012;7(10):e47259. doi: 10.1371/journal.pone.0047259. Epub 2012 Oct 22. 10.1371/journal.pone.0047259 PubMed 23110063
Map uploaded by the depositor.