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pSAM
(Plasmid #45174)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45174 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5739
  • Modifications to backbone
    The original pBR322 Origin of Replication from pET-28a(+) was replaced with the p15A Origin of Replication.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis/Thrombin/T7 (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5' - TAA TAC GAC TCA CTA TAG GG - 3'
  • 3′ sequencing primer 5' - GCT AGT TAT TGC TCA GCG G - 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

In Sathiamoorthy et al, there is an error in the title of one of the supplementary figures. Figure S3 should read "Construction of pSAMRNAI" and in the figure the final constructed vector is "pSAMRNAI".

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSAM was a gift from Jumi Shin (Addgene plasmid # 45174 ; http://n2t.net/addgene:45174 ; RRID:Addgene_45174)
  • For your References section:

    Boundaries of the origin of replication: creation of a pET-28a-derived vector with p15A copy control allowing compatible coexistence with pET vectors. Sathiamoorthy S, Shin JA. PLoS One. 2012;7(10):e47259. doi: 10.1371/journal.pone.0047259. Epub 2012 Oct 22. 10.1371/journal.pone.0047259 PubMed 23110063