pSAM
(Plasmid #45174)

• Purpose: (Empty Backbone)
• Depositing Lab: Jumi Shin
• Publication: Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.1371/journal.pone.0047259. Epub 2012 Oct 22.

Backbone

• Vector backbone: pET-28a(+)
• Backbone manufacturer: Novagen
• Backbone size (bp): 5739
• Modifications to backbone: The original pBR322 Origin of Replication from pET-28a(+) was replaced with the p15A Origin of Replication.
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis/Thrombin/T7 (N terminal on backbone)
  • 6xHis (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5' - TAA TAC GAC TCA CTA TAG GG - 3'
• 3′ sequencing primer: 5' - GCT AGT TAT TGC TCA GCG G - 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In Sathiamoorthy et al, there is an error in the title of one of the supplementary figures. Figure S3 should read "Construction of pSAMRNAI" and in the figure the final constructed vector is "pSAMRNAI".