pCRII-Topo Vglut2 in situ probe
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45639||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4500
Vector typein situ
Growth in Bacteria
Gene/Insert nameVglut2 in situ probe
Alt namesolute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
SpeciesM. musculus (mouse)
Insert Size (bp)517
Mutationfragment contains nt 2477-2993 of slc17a6 (Vglut2) mRNA sequence (GenBank ID AK045409.1)
Entrez GeneSlc17a6 (a.k.a. 2900073D12Rik, DNPI, VGLUT2)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For in vitro transcription, use the SpeI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-Topo Vglut2 in situ probe was a gift from Jeffrey Macklis (Addgene plasmid # 45639 ; http://n2t.net/addgene:45639 ; RRID:Addgene_45639)
Map uploaded by the depositor.