pCRII-Topo Vglut2 in situ probe
(Plasmid #45639)

• Depositing Lab: Jeffrey Macklis
• Publication: Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. doi: 10.1038/nn.2387. Epub 2009 Aug 5.

Backbone

• Vector backbone: pCRII-Topo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4500
• Vector type: in situ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vglut2 in situ probe
• Alt name: Vglut2
• Alt name: Slc17a6
• Alt name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• Species: M. musculus (mouse)
• Insert Size (bp): 517
• Mutation: fragment contains nt 2477-2993 of slc17a6 (Vglut2) mRNA sequence (GenBank ID AK045409.1)
• GenBank ID: AK045409.1
• Entrez Gene: Slc17a6 (a.k.a. 2900073D12Rik, DNPI, VGLUT2)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 Rev, Sp6
• 3′ sequencing primer: T7, M13 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For in vitro transcription, use the SpeI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.