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pCRII-Topo Vglut2 in situ probe
(Plasmid #45639)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45639 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCRII-Topo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4500
  • Vector type
    in situ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vglut2 in situ probe
  • Alt name
    Vglut2
  • Alt name
    Slc17a6
  • Alt name
    solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    517
  • Mutation
    fragment contains nt 2477-2993 of slc17a6 (Vglut2) mRNA sequence (GenBank ID AK045409.1)
  • GenBank ID
    AK045409.1
  • Entrez Gene
    Slc17a6 (a.k.a. 2900073D12Rik, DNPI, VGLUT2)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

For in vitro transcription, use the SpeI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Topo Vglut2 in situ probe was a gift from Jeffrey Macklis (Addgene plasmid # 45639 ; http://n2t.net/addgene:45639 ; RRID:Addgene_45639)