Addgene - pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP Plasmid Data

Plasmid 35501: pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Gene/insert name:
hChR2
Insert size:
1661
Species:
Chlamydomonas reinhardtii
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
C128S and D156A
Vector backbone:
pAAV
(Search Vector Database)
Vector type:
Mammalian Expression ; AAV
Backbone size w/o insert (bp):
5385
Modifications to Backbone:
Addition of a CaMKIIa promoter and a WPRE
Promoter:
CaMKIIa
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AGTCCTGCAGTATTGTGTAT List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Comments:

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Neocortical excitation/inhibition balance in information processing and social dysfunction. Yizhar et al (Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35501" in your Materials and Methods section.