|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35501||Plasmid sent as bacteria in agar stab||1||$65||Add to Cart|
- Backbone size w/o insert (bp) 5385
Modifications to backboneAddition of a CaMKIIa promoter and a WPRE
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1661
MutationC128S and D156A
- Promoter CaMKIIa
/ Fusion Protein
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer AGTCCTGCAGTATTGTGTAT
- 3′ sequencing primer GCAATAGCATGATACAAAGG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 35501)
For your References section:Neocortical excitation/inhibition balance in information processing and social dysfunction. Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K. Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. 10.1038/nature10360 PubMed 21796121
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.