Addgene - pAAV-CaMKIIa-hChR2(H134R)-EYFP Plasmid Data

Plasmid 26969: pAAV-CaMKIIa-hChR2(H134R)-EYFP

Gene/insert name:
hChR2(H134R)
Alt name:
ChR2
Insert size:
1662
Species:
H. sapiens (human)
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
Histidine 134 is mutated to Arginine
Vector backbone:
pAAV
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5390
Cloning site 5':
BamHI, SalI, KpnI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI, HinDIII
Site destroyed during cloning:
No
5' sequencing primer:
ctcagaagccccaagctcgtc List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3 (rec A-) cells to avoid recombinations with the LTRs
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Comments:

This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.

For additional information please visit - http://www.optogenetics.org

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Global and local fMRI signals driven by neurons defined optogenetically by type and wiring. Lee et al (Nature. 2010 Jun 10. 465(7299):788-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26969" in your Materials and Methods section.