Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

hE-cadherin/α-catenin fusion-pcDNA3
(Plasmid #45771)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45771 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 10000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hE-cadherin/Xe α-catenin fusion
  • Alt name
    human E-cadherin
  • Alt name
    Xenopus α-catenin
  • Species
    H. sapiens (human), X. laevis (frog)
  • Insert Size (bp)
    4600
  • Mutation
    Human E-cadherin (aa 1-801) fused to Xenopus α-catenin (aa 201-end)
  • GenBank ID
    NM_004360.3; NP_004351.1 NM_001090631.1; NP_001084100.1
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Entrez Gene
    ctnna1 (a.k.a. cap102, catna1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGH-rev; Sp6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT)
  • Terms and Licenses

Depositor Comments

For this E-cadherin–α-catenin fusion construct that lacks the β-catenin binding domain in both E-cadherin and α-catenin, cDNA encoding the membrane proximal region of the cadherin cytoplasmic domain was amplified between nucleotides 1760 and 2530 using
5′ (5′-TGAGCACGTGAAGAACAGCACGTACAC-3′) and
3′ (5′-CCCTGGCCATTTCCAATTTCATCGGGA-3′)
oligonucleotide primers, and the resultant 770-bp fragment was cloned into a Xenopus α-catenin cDNA such that amino acid 809 in E-cadherin was fused in frame with amino acid 201 in α-catenin (E-cad-DEIGN/GHRDQ-α-catenin). This fusion region was further subcloned into the E-cad/pcDNA3 plasmid (Addgene plasmid 45769) to make the E-cadherin–α-catenin cDNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hE-cadherin/α-catenin fusion-pcDNA3 was a gift from Barry Gumbiner (Addgene plasmid # 45771 ; http://n2t.net/addgene:45771 ; RRID:Addgene_45771)
  • For your References section:

    E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. Gottardi CJ, Wong E, Gumbiner BM. J Cell Biol. 2001 May 28;153(5):1049-60. 10.1083/jcb.153.5.1049 PubMed 11381089