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IL2R/hE-cadherin-cytotail
(Plasmid #45773)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45773 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL2R/hE-cadherin-cytotail
  • Alt name
    Interleukin-2 receptor α subunit extracellular and transmembrane domains
  • Alt name
    E-cadherin cytoplasmic domain
  • Species
    H. sapiens (human)
  • Mutation
    Interleukin-2 receptor α subunit extracellular and transmembrane domains fused to E-cadherin cytoplasmic domain
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT). IL2R-1 α subunit cDNA from pCMV-IL2R-1 from Susan LaFlamme (Albany Medical College, Albany, NY), originally from Anthony Giordano (Giordano et al ., 1991)
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

This IL2R/E-cadherin cytoplasmic chimera fuses the extracellular and transmembrane domains of the interleukin-2 receptor α subunit to the cytoplasmic domain of E-cadherin. This construct can bind β-catenin but cannot engage in homophilic adhesive activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL2R/hE-cadherin-cytotail was a gift from Barry Gumbiner (Addgene plasmid # 45773 ; http://n2t.net/addgene:45773 ; RRID:Addgene_45773)
  • For your References section:

    E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. Gottardi CJ, Wong E, Gumbiner BM. J Cell Biol. 2001 May 28;153(5):1049-60. 10.1083/jcb.153.5.1049 PubMed 11381089