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a1Ha-pcDNA3
(Plasmid #45809)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45809 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 13000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    a1Ha
  • Alt name
    CACNA1H
  • Alt name
    Cav3.2a
  • Alt name
    α1H, Hh8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7800
  • GenBank ID
    AF051946
  • Entrez Gene
    CACNA1H (a.k.a. CACNA1HB, Cav3.2, ECA6, EIG6, HALD4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains nt −74 to 7461 of α1H
5' end cloned into EcoRV site of pcDNA3: GATATCATCGATGAATTC then CGGGCGGTG as reported in Genbank AF051946.
3' ends same place as Genbank entry (CTCCCCTCCG), then a bit of the PUC18 polylinker, before the XbaI site of pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    a1Ha-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45809 ; http://n2t.net/addgene:45809 ; RRID:Addgene_45809)
  • For your References section:

    Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family. Cribbs LL, Lee JH, Yang J, Satin J, Zhang Y, Daud A, Barclay J, Williamson MP, Fox M, Rees M, Perez-Reyes E. Circ Res. 1998 Jul 13;83(1):103-9. 10.1161/01.RES.83.1.103 PubMed 9670923