|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45809||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 13000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt nameα1H, Hh8
SpeciesH. sapiens (human)
Insert Size (bp)7800
Entrez GeneCACNA1H (a.k.a. CACNA1HB, Cav3.2, ECA6, EIG6, HALD4)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
contains nt −74 to 7461 of α1H
5' end cloned into EcoRV site of pcDNA3: GATATCATCGATGAATTC then CGGGCGGTG as reported in Genbank AF051946.
3' ends same place as Genbank entry (CTCCCCTCCG), then a bit of the PUC18 polylinker, before the XbaI site of pcDNA3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:a1Ha-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45809 ; http://n2t.net/addgene:45809 ; RRID:Addgene_45809)
For your References section:Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family. Cribbs LL, Lee JH, Yang J, Satin J, Zhang Y, Daud A, Barclay J, Williamson MP, Fox M, Rees M, Perez-Reyes E. Circ Res. 1998 Jul 13;83(1):103-9. 10.1161/01.RES.83.1.103 PubMed 9670923