pCM62
(Plasmid #45826)

• Purpose: (Empty Backbone)
• Depositing Lab: Mary Lidstrom
• Publication: Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: NEB
• Backbone size (bp): 6878
• Modifications to backbone: The 2.0 kb NdeI/NotI region of pDN19X containing the traJ' allele that allowed efficient replication and/or transfer in M. extorquens AM1 was transferred into the corresponding region of pTJS75 (Schmidhauser & Helinski, 1985), a parent vector of pDN19 (Nunn et al., 1990), to create pCM48. The plasmid pCM48 was then cut with MunI and BlnI, and the DNA ends were blunted and self-ligated to produce pCM50. A 5.3 kb HindIII/XmnI region of pCM50 was ligated with the blunted 1.9 kb AatII/AvaII region of pUC19(Yanisch-Perron et al., 1985) to make pCM62. During the construction of pCM62 an additional 356 bp inward from the site of XmnI cleavage of pCM50 was lost, presumably caused by either non-specific cleavage or by the exonuclease activity of T4 DNA polymerase.
• Vector type: broad host range cloning vector
• Promoter: lac

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector is a broad host range vector (bhr) and is used in the Methylobacterium extorquens AM1. Also can be maintained in the a-proteo-bacteria Agrobacterium tumefaciens, Methylobacterium strains CM4 and DM4, and Rhodobacter sphaeroides, the b-proteobacterium Ralstonia eutropha and the c-proteobacteria Methylococcus capsulatus Bath and Pseudomonas aeruginosa (see linked article).