Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCM66
(Plasmid #45827)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45827 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCM62
  • Backbone manufacturer
    Lidstrom Lab (Addgene plasmid 45826)
  • Backbone size (bp) 7607
  • Modifications to backbone
    pCM66 was constructed by inserting the 1.3 kb HincII fragment of pUC4K (Veira&Messing, 1982) between the EcoRV and NruI sites of pCM62 (Addgene plasmid 45826) to add kanamycin resistance.
  • Vector type
    broad host range cloning vector in bacteria
  • Promoter lac

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Vector is a broad host range vector (bhr) and is used in the Methylobacterium extorquens AM1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM66 was a gift from Mary Lidstrom (Addgene plasmid # 45827 ; http://n2t.net/addgene:45827 ; RRID:Addgene_45827)
  • For your References section:

    Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985