|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45827||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLidstrom Lab (Addgene plasmid 45826)
- Backbone size (bp) 7607
Modifications to backbonepCM66 was constructed by inserting the 1.3 kb HincII fragment of pUC4K (Veira&Messing, 1982) between the EcoRV and NruI sites of pCM62 (Addgene plasmid 45826) to add kanamycin resistance.
Vector typebroad host range cloning vector in bacteria
- Promoter lac
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Vector is a broad host range vector (bhr) and is used in the Methylobacterium extorquens AM1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM66 was a gift from Mary Lidstrom (Addgene plasmid # 45827 ; http://n2t.net/addgene:45827 ; RRID:Addgene_45827)
For your References section:Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985