|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45863||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLidstrom Lab (Addgene plasmid 45826)
- Backbone size w/o insert (bp) 6878
- Total vector size (bp) 7978
Vector typeBacterial Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecre recombinase
Alt namecyclization recombinase
SpeciesEnterobacteria phage P1
Insert Size (bp)1100
- Promoter lac
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid.
Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM157 was a gift from Mary Lidstrom (Addgene plasmid # 45863 ; http://n2t.net/addgene:45863 ; RRID:Addgene_45863)
For your References section:Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx CJ, Lidstrom ME. Biotechniques. 2002 Nov;33(5):1062-7. 10.2144/02335rr01 PubMed 12449384