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pCM157
(Plasmid #45863)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45863 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCM62
  • Backbone manufacturer
    Lidstrom Lab (Addgene plasmid 45826)
  • Backbone size w/o insert (bp) 6878
  • Total vector size (bp) 7978
  • Vector type
    Bacterial Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cre recombinase
  • Alt name
    cyclization recombinase
  • Alt name
    cre
  • Species
    Enterobacteria phage P1
  • Insert Size (bp)
    1100
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid.
Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM157 was a gift from Mary Lidstrom (Addgene plasmid # 45863 ; http://n2t.net/addgene:45863 ; RRID:Addgene_45863)
  • For your References section:

    Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx CJ, Lidstrom ME. Biotechniques. 2002 Nov;33(5):1062-7. 10.2144/02335rr01 PubMed 12449384