pOSIP-KO (KanR, 186)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45985||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6472
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth instructionsContains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.
Copy numberHigh Copy
- 5′ sequencing primer TAAACTGCCAGGAATTGGGGATC
- 3′ sequencing primer GAAACGCAAAAAGGCCATCCGTC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that there is a E59G mutation in Integrase 186.
St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOSIP-KO (KanR, 186) was a gift from Drew Endy & Keith Shearwin (Addgene plasmid # 45985 ; http://n2t.net/addgene:45985 ; RRID:Addgene_45985)
For your References section:One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148