pQUASp-mCD8GFP
(Plasmid
#46163)
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PurposeExpression of murine CD8GFP in insects
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 46163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepQUASp
- Backbone size w/o insert (bp) 9896
- Total vector size (bp) 11285
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Vector typeInsect Expression
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Selectable markerswhite
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCD8GFP
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Alt namemouseCD8
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Alt nameGFP
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Alt nameCD8
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)1389
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer TTTGAAAACCGGTGATAGAGCCTG
- 3′ sequencing primer ACCAGGGGATGCTTAATTGTGTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQUASp-mCD8GFP was a gift from Christopher Potter (Addgene plasmid # 46163 ; http://n2t.net/addgene:46163 ; RRID:Addgene_46163)