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pET15b/GgVcl 884-1066 (aka Vt)
(Plasmid #46176)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46176 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6260
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl 884-1066
  • Alt name
    Vinculin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    555
  • Mutation
    aa 884-1066 (tail domain)
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GgVcl 884-1066 (aka Vt) was a gift from Susan Craig (Addgene plasmid # 46176 ; http://n2t.net/addgene:46176 ; RRID:Addgene_46176)
  • For your References section:

    Polyphosphoinositides inhibit the interaction of vinculin with actin filaments. Steimle PA, Hoffert JD, Adey NB, Craig SW. J Biol Chem. 1999 Jun 25;274(26):18414-20. 10.1074/jbc.274.26.18414 PubMed 10373448