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pET15b/ECFP-GgVcl 884-1066 (Vt)
(Plasmid #46180)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46180 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7013
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl 884-1066
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    555
  • Mutation
    aa 884-1066 (tail domain)
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • ECFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/ECFP-GgVcl 884-1066 (Vt) was a gift from Susan Craig (Addgene plasmid # 46180 ; http://n2t.net/addgene:46180 ; RRID:Addgene_46180)