pMXs-hM₃O-IP
(Plasmid #46645)

• Depositing Lab: Nobuaki Kikyo
• Publication: Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.

Backbone

• Vector backbone: pMXs-IP
• Backbone manufacturer: Toshio Kitamura lab, University of Tokyo (PMID: 14585362)
• Backbone size w/o insert (bp): 5847
• Total vector size (bp): 7133
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hM₃O
• Alt name: human Oct3/4
• Alt name: Pou5f1
• Alt name: octamer-binding transcription factor 4
• Species: H. sapiens (human)
• Insert Size (bp): 1286
• Mutation: contains a fragment of human MyoD (aa1-62) fused to the N-terminus of full length human Oct3/4
• GenBank ID: NM_002701.4 NP_002692.2
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Tags / Fusion Proteins:
  • human MyoD (aa 1-62) (N terminal on insert)
  • IRES Puro (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBMN 5' (5'-GCTTGGATACACGCCGC-3'); pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
• 3′ sequencing primer: pCDH-rev (5'-GCATTCCTTTGGCGAGAG-3'); puro-variant-R (5'-gtgggcttgtactcggtcat-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry