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Plasmid 26817: pcDNA3.3_SOX2
  • 5'UTR-SOX2-3'UTR

  • SOX2

  • 1137

  • H. sapiens (human)

  • SOX2 (ANOP3, MCOPS3)

  • pcDNA3.3
    (Search Vector Database)

  • Mammalian Expression

  • 5400

  • CMV Forward List of Sequencing Primers

  • TK polyA reverse

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Change to mRNA reprogramming protocol (application/x-pdf)

  • Derrick Rossi

  • MTA

Comments: 

Note that SOX2 has some silent mutations.

Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren et al (Cell Stem Cell. 2010 Sep 29. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26817" in your Materials and Methods section.