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(Plasmid #26817)

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Available to Academic and Nonprofits Only


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer TK polyA reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note that SOX2 has some silent mutations.

Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3_SOX2 was a gift from Derrick Rossi (Addgene plasmid # 26817)
  • For your References section:

    Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Cell Stem Cell. 2010 Sep 29. ():. 10.1016/j.stem.2010.08.012 PubMed 20888316