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Plasmid 26818: pcDNA3.3_c-MYC
  • 5'UTR-c-MYC-3'UTR

  • c-myc

  • 1504

  • H. sapiens (human)

  • MYC (MRTL, MYCC, bHLHe39, c-Myc)

  • Sequence begins from second ATG

  • pcDNA3.3
    (Search Vector Database)

  • Mammalian Expression

  • 5400

  • CMV Forward List of Sequencing Primers

  • TK polyA reverse

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • Change to mRNA reprogramming protocol (application/x-pdf)

  • Derrick Rossi

  • MTA

Comments: 

Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

Comment from lab: "with reference to NM_002467.4 sequence we have two mutations at
following positions: 172 (A to G) and 174 (C to G)
there is some confusion as to what the ref seq is exactly but these are the constructs that we've been using successfully."

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren et al (Cell Stem Cell. 2010 Sep 29. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26818" in your Materials and Methods section.