pABpuro-BluF
(Plasmid #46824)

• Purpose: luciferase reporter for BmalI promoter
• Depositing Lab: Steven Brown
• Publication: Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 2005 Sep 27.

Backbone

• Vector backbone: pWPI
• Backbone manufacturer: Addgene plasmid 12254
• Modifications to backbone: The EF1α promoter and gfp gene were removed from lentiviral backbone plasmid pWPI.
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmal1
• Alt name: Arntl
• Alt name: aryl hydrocarbon receptor nuclear translocator-like
• Species: M. musculus (mouse)
• Entrez Gene: Bmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
• Promoter: Bmal1
• Tag / Fusion Protein:
  • luciferase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: LucNRev
• 3′ sequencing primer: pBABE 3'

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert consists of 1 kb of mouse Bmal1 upstream region and 53 nucleotides of exon 1, fused in-frame to the luciferase coding region, and followed by 1 kb of Bmal1 3′UTR.