Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

psd44-GqQL
(Plasmid #46826)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46826 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psd44
  • Backbone manufacturer
    Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
  • Modifications to backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    g protein alpha q
  • Alt name
    GNAQ
  • Alt name
    guanine nucleotide binding protein (G protein), q polypeptide
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1120
  • Mutation
    Q209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
  • Entrez Gene
    GNAQ (a.k.a. CMC1, G-ALPHA-q, GAQ, SWS)
  • Promoter PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer hPGK-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA0Q000C0).

Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psd44-GqQL was a gift from Agnese Mariotti (Addgene plasmid # 46826 ; http://n2t.net/addgene:46826 ; RRID:Addgene_46826)