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psd44-G13QL
(Plasmid #46828)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46828 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psd44
  • Backbone manufacturer
    Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
  • Backbone size w/o insert (bp) 9195
  • Total vector size (bp) 10370
  • Modifications to backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GNA13
  • Alt name
    guanine nucleotide binding protein (G protein), alpha 13
  • Alt name
    G protein alpha 13
  • Alt name
    G alpha 13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1175
  • Mutation
    Q226L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
  • Entrez Gene
    GNA13 (a.k.a. G13, HG1N)
  • Promoter PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer hPGK-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA13000C0).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psd44-G13QL was a gift from Agnese Mariotti (Addgene plasmid # 46828 ; http://n2t.net/addgene:46828 ; RRID:Addgene_46828)