psd44-Lactadherin46
(Plasmid #46830)

• Purpose: expression of human lactadherin in mammalian cells
• Depositing Lab: Agnese Mariotti
• Publication: Mariotti lab plasmids (unpublished)

Backbone

• Vector backbone: psd44
• Backbone manufacturer: Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
• Backbone size w/o insert (bp): 9195
• Total vector size (bp): 10370
• Modifications to backbone: pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MFGE8
• Alt name: milk fat globule-EGF factor 8 protein
• Alt name: lactadherin
• Alt name: BA46
• Species: H. sapiens (human)
• Insert Size (bp): 1196
• GenBank ID: NM_005928
• Entrez Gene: MFGE8 (a.k.a. BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47)
• Promoter: PGK promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: hPGK-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Originally from RSPD, Germany, plasmid IOH46424, in pDEST26 as backbone.