Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGL3-24p3/LCN2-282C/EBP mut-LUC
(Plasmid #46868)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46868 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5100
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    24p3/lipocalin 2 proximal promoter
  • Alt name
    LCN2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    282
  • Mutation
    CEBP site is mutated (see comments)
  • Promoter 24p3 promoter (inactive)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (not destroyed)
  • 3′ cloning site Xho I (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Primers used to create the C/EBP mutation were (lower case are mutations):
5-CAG CCC TTC CTG gca CTt ggC CTT GCA CAG TTC CGA C-3 and
5-AAC TGT GCA AGG cca AGt gcC AGG AAG GGC TGC AGG G-3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-24p3/LCN2-282C/EBP mut-LUC was a gift from Sarah Gaffen (Addgene plasmid # 46868 ; http://n2t.net/addgene:46868 ; RRID:Addgene_46868)
  • For your References section:

    Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598