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pGL3-24p3/LCN2-Luc
(Plasmid #25463)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25463 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    24p3/lipocalin 2 proximal promoter
  • Alt name
    LCN2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1438
  • Mutation
    none
  • Entrez Gene
    Lcn2 (a.k.a. 24p3, AW212229, NRL, Sip24)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer RVprimer 3
  • 3′ sequencing primer GLprimer2
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-24p3/LCN2-Luc was a gift from Sarah Gaffen (Addgene plasmid # 25463 ; http://n2t.net/addgene:25463 ; RRID:Addgene_25463)
  • For your References section:

    Cytokines link osteoblasts and inflammation: microarray analysis of interleukin-17- and TNF-alpha-induced genes in bone cells. Shen F, Ruddy MJ, Plamondon P, Gaffen SL. J Leukoc Biol. 2005 Mar . 77(3):388-99. 10.1189/jlb.0904490 PubMed 15591425