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hGFAP-fLuc
(Plasmid #40589)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40589 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hGFAP promoter
  • Alt name
    hGFAP
  • Alt name
    GFAP promoter
  • Alt name
    GFAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    Contains human GFAP promoter fragment (-2163 to +47). ATG codon at position +15 changed to TTG (transcription starts at the firefly luciferase ATG at position +102)
  • Promoter GFAP promoter
  • Tag / Fusion Protein
    • Firefly luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The 2.2 kb human GFAP promoter fragment was derived from the GFAP-lacZ transgene previously described by Brenner et al. (1994).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

To construct a transgene expressing firefly luciferase regulated by the GFAP promoter (GFAP-fLuc), the depositor utilized the 2.2 kb human GFAP promoter derived from the GFAP-lacZ transgene previously described by Brenner et al. (1994). The GFAP promoter fragment (-2163 to +47) was subcloned into the Bgl II site in the pGL-3 basic vector (Promega, Madison, WI, USA) containing the firefly luciferase gene. The transgene was sequenced to confirm correct orientation and sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hGFAP-fLuc was a gift from Albee Messing (Addgene plasmid # 40589 ; http://n2t.net/addgene:40589 ; RRID:Addgene_40589)
  • For your References section:

    Dual transgenic reporter mice as a tool for monitoring expression of glial fibrillary acidic protein. Cho W, Hagemann TL, Johnson DA, Johnson JA, Messing A. J Neurochem. 2009 Jul;110(1):343-51. Epub 2009 May 5. 10.1111/j.1471-4159.2009.06146.x PubMed 19457099