FU-MAP2-Gateway
(Plasmid #43915)

• Purpose: (Empty Backbone)
• Depositing Lab: John Gearhart
• Publication: Addis et al PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9.

Backbone

• Vector backbone: FUdeltaGW-rtTA
• Backbone manufacturer: Addgene plasmid 19780
• Backbone size (bp): 9600
• Modifications to backbone: Ubiquitin promoter-rtTA sequence removed. Human MAP2 promoter sequence from pGZ-hMAP2 and Gateway casette from pEF-DEST51 inserted.
• Vector type: Mammalian Expression, Lentiviral ; Gateway Destination vector
• Promoter: Human MAP2 promoter
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • non-standard His tag (HHRHHH) (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Use ccdb-resistant cells, such as OneShot Survival ccdb Competent Cells (Invitrogen)
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • FU-MAP2-Gateway annotated genbank file
• A portion of this plasmid was derived from a plasmid made by: Backbone plasmid FUdeltaGW-rtTA from Addgene (Plasmid #19780, Konrad Hochedlinger lab); Human MAP2 promoter sequence from plasmid pGZ-hMAP2 (SR10047PA-1) from System Biosciences (Mountain View, CA); Gateway cassette from pEF-DEST51 from Invitrogen.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway.