|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32401||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneCX lentiviral vector
Modifications to backboneinsertion of the whole nestin promoter-GFP-nestin enhancer and betaglobin polyA into a CX lentiviral vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namenestin promoter/enhancer GFP
Insert Size (bp)5900
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer gacagagacagatccattc (Common Sequencing Primers)
Terms and Licenses
Note that there are some discrepancies between Addgene's QC sequence and the depositor's assembled sequence. The plasmid should still function as reported.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CX-nestinGFPas was a gift from Patrick Salmon & Henryk Zulewski (Addgene plasmid # 32401 ; http://n2t.net/addgene:32401 ; RRID:Addgene_32401)
For your References section:Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. Eberhardt M, Salmon P, von Mach MA, Hengstler JG, Brulport M, Linscheid P, Seboek D, Oberholzer J, Barbero A, Martin I, Muller B, Trono D, Zulewski H. Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May 11. 10.1016/j.bbrc.2006.05.016 PubMed 16713999