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C3 luc (T1 del)
(Plasmid #11358)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11358 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 6688
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C3 promoter
  • Alt name
    C3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Entrez Gene
    C3 (a.k.a. AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer lucNrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C3 luc (T1 del) was a gift from Donald McDonnell (Addgene plasmid # 11358 ; http://n2t.net/addgene:11358 ; RRID:Addgene_11358)
  • For your References section:

    Identification of the sequences within the human complement 3 promoter required for estrogen responsiveness provides insight into the mechanism of tamoxifen mixed agonist activity. Fan JD, Wagner BL, McDonnell DP. Mol Endocrinol. 1996 Dec . 10(12):1605-16. 10.1210/mend.10.12.8961270 PubMed 8961270