C3 luc (T1 del)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11358||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6688
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameC3 promoter
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GeneC3 (a.k.a. AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p)
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer lucNrev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:C3 luc (T1 del) was a gift from Donald McDonnell (Addgene plasmid # 11358 ; http://n2t.net/addgene:11358 ; RRID:Addgene_11358)
For your References section:Identification of the sequences within the human complement 3 promoter required for estrogen responsiveness provides insight into the mechanism of tamoxifen mixed agonist activity. Fan JD, Wagner BL, McDonnell DP. Mol Endocrinol. 1996 Dec . 10(12):1605-16. 10.1210/mend.10.12.8961270 PubMed 8961270
Map uploaded by the depositor.