Addgene - C3 luc (T1 del) Plasmid Data

Plasmid 11358: C3 luc (T1 del)

Gene/insert name:
C3 promoter
Alt name:
C3
Insert size:
1100
Species:
H. sapiens (human)
Entrez Gene:
C3 (AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1)
Vector backbone:
na
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
6688
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
lucNrev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Donald McDonnell
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of the sequences within the human complement 3 promoter required for estrogen responsiveness provides insight into the mechanism of tamoxifen mixed agonist activity. Fan et al (Mol Endocrinol. 1996 Dec . 10(12):1605-16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11358" in your Materials and Methods section.