Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

STAT3(Y705F)-TAL-Luc
(Plasmid #46933)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46933 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTAL-Luc
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 2351
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT3(Y705F)
  • Alt name
    STAT3
  • Alt name
    signal transducer and activator of transcription 3 (acute-phase response factor)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2351
  • Mutation
    Tyrosine 705 to Phenylalanine
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Promoter TA Luciferase
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5'-CGGGAGGTACTTGGAGCG-3'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STAT3(Y705F)-TAL-Luc was a gift from Afshin Dowlati (Addgene plasmid # 46933 ; http://n2t.net/addgene:46933 ; RRID:Addgene_46933)
  • For your References section:

    The association and nuclear translocation of the PIAS3-STAT3 complex is ligand and time dependent. Dabir S, Kluge A, Dowlati A. Mol Cancer Res. 2009 Nov;7(11):1854-60. doi: 10.1158/1541-7786.MCR-09-0313. Epub 2009 Nov 10. 10.1158/1541-7786.MCR-09-0313 PubMed 19903771